User Guide

Welcome to Poriscope’s user guide. This guide will walk you through the main features and functionality of the application.

Sections

• Getting Started
  • Prerequisites
  • Installing the Stable Version
  • Launching Poriscope
  • Documentation
• User Installation
  • Prerequisites
  • Stable Release (Recommended)
  • Latest Unreleased Version (Advanced)
  • Launching Poriscope
• Workflow Overview
  • 1. Raw Data
  • 2. Event Analysis
  • 3. Metadata Handling
  • 4. Clustering
• Tutorial and Documentation
• Raw Data Tab
  • Step 1: Loading Your Data
  • Step 2: Applying a Filter
  • Step 3: Finding Events
  • Step 4: Writing Events
• Event Analysis Tab
  • Step 1: Load Event Database
  • Step 2: Apply a Filter
  • Step 3: Fit Events
  • Step 4: Write to Database
• MetaData Tab
  • Step 1: Load Metadata Database
  • Step 2: Choose Plot Type and Configure Axes
  • Step 3: Apply Filters
  • Step 4: Generate and Export
• Clustering Tab
  • Step 1: Load Metadata Database
  • Step 2: Configure Clustering Settings
  • Step 3: Merge Clusters (Optional)
  • Step 4: Commit Cluster Labels
  • Step 5: Export Plot Data
• Protein Tab
  • Step 1: Load a Fitted Events Database
  • Step 2: Choose Scope
  • Step 3: Enter Pore Geometry
  • Step 4: Choose a Fitting Mode
  • Step 5: Configure Sampling and Binning
  • Step 6: Generate the Fit
  • Step 7a: Commit Individual Fits
  • Step 7b: Report Ensemble Fit
  • Step 8: Apply Filters
  • Step 9: Export Plot Data
  • Step 10: Inspect Individual Events
• Settings Tab
  • General Tab
  • Advanced Settings Tab
  • About Tab
• Menus and Session Management
  • Session Options Overview
  • Settings Access
  • Data Menu
  • Analysis Menu
  • Help Menu
• Scripting with Poriscope
  • Setting up our workspace
  • Loading raw data
  • Create a filter
  • Finding events
  • Saving an event database
  • Loading an event database
  • Fitting events
  • Saving event metadata
  • Loading event metadata
• Filtering and Querying
  • Filter Modes
  • Creating a Filter
  • Editing a Filter
  • Saving and Loading Filters
  • Common Mistakes
  • Available Columns
• Running the Software
  • Purpose of the Running the Software Section
  • Relationship to the Tutorial Series
  • Launching Poriscope
  • Loading Data
  • Visualizing a Trace
  • Filtering the Signal
  • Event Finding
  • Channel Selection Notes
  • Example
  • Writing Results
• FAQ
  • I loaded a file, but nothing appeared. What happened?
  • I’m fitting my events, but it shows the fit from a previous dataset.
  • I clicked Analysis -> New Analysis Tab -> EventAnalysisController or RawDataController, but nothing changes.
  • I’m trying to load a database into Metadata View or Event Analysis View, but it says the format is unsupported.
  • Is Linux supported?
  • Where can I find the data used in the tutorials?